u/xmflrtm

hello, i already did my primer efficiency and will be performing my final qpcr run soon. I need help in regard with the data I need to show in my R&D. I want to know which genes are jn my sample and how much they’re expressed. What data visualizations, analysis and discussions should I be able to provide? Just to make sure… Thank u :) appreciate ur help

here’s what i’m gonna put but maybe i missed some. pls suggest:

• amplification/melt curves by software
• ddCt or 2-ddCt (not sure which one)
• linearity
• box plots

preferably someone with experience in qPCR, bioinformatics, or similar. i will provide raw primer efficiency data.

i’m trying too hire someone and i’m just a student. i saved up for this money i’m about to pay but i’m hesitating because i might get scammed. how will I know if I can trust my freelancer in Upwork? I haven’t started my contract yet because payment is not working and freelancer suggested I pay outside upwork… Help me guys