u/troyotahilux

I ran my extracted DNA through a spectrophotometer. While the A260/A280 reading showed great results (1.84, 1.92, and 2.01), my A260/230 had a very low reading (0.35, 0.43, and 0.35). I used the Qiagen FAST Stool Mini Kit for the extraction, and I did read that low concentration of elution buffer can affect this. The protocol states that the elution buffer to be used should be at 200ul; however, my laboratory instructor suggested using only 50ul.

I am a biology undergrad. Your help/explanation would be appreciated!