Staining reference controls in u bottom plates for spectral flow
Has anyone successfully stained reference controls in something like a 96-well plate instead of tubes for higher parameter panels?
I’m currently running 13–20 color panels on our BD A8, stain my primary samples in 96-well plates and my reference controls separately in tubes simultaneously during the same protocol. However, the mix of both substantially slows down prep, especially when processing multiple tissue types or staining multiple panels.
As I'm building higher parameter panels, I’m considering staining the reference controls in plates as well. My main concern is potential cross-well contamination/spillover during wash steps (ex from flicking) subtly altering the spectra and impact unmixing accuracy. I have tried this with some success in smaller panels, but as I develop higher parameter panels I'm a bit paranoid.
Any advice/input is appreciate. I'm curious if anyone has any workflows that have worked well for them.
Thank you!