u/sunnycloak

Hi! I am quite new to flow cytometry and need to titrate a new antibody for our panel. From what I've read, it seems important to titrate on the same cell numbers one would have in the real samples for representative level of antigen saturation. Would it be incorrect to use much smaller cell numbers, in the interest of saving samples? We usually don't use a set number of cells for flow either, but run the whole samples, which can differ in number of cells.

Apologies if this has been asked already in the subreddit. Many thanks!