Question about purposely creating a Proton out of codon alignment
Former viral researcher here, out of the lab for 20 yrs. Reading a book about Covid 19 and Wuhan. Keep seeing the furin cleavage site controversy. Proponents of the natural covid evolution stress that the furin cleavage site is out of frame, so if they engineered covid 19, why would they sloppily make a coding sequence out of frame? But what if they did that on purpose, so they they made the entire plasmid ready to go except for one codon. Then when they did pcr, one primer had the correct codon sequence. They could use for example 80 bases in the primer, correct the sequence in the middle and the primer would still bind even if one or more bases are misaligned in the middle. Possible?