u/hana-maki

what can cause a sample dye front to be discreet (not diffusing from wells) vs continuous?

what can cause a sample dye front to be discreet (not diffusing from wells) vs continuous?

i remade both my sample buffer and tris-gly PAGE buffer using the recipe from sigma products online but i still am getting discreet fronts sometimes where the sample dye doesn’t really diffuse out of the wells.

is that a cause of concern?

thanks!

u/hana-maki — 11 hours ago