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u/elixirelyse

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Hi everyone! For our assay, we’re required to count the number of crystals formed (aggregates) per image. However, when we do the Analyze Particles function, it “breaks up” the crystal instead of just counting the whole thing as one. Things we’ve done to the picture: 

  1. Original Image

  2. Image > Type > 8-bit

  3. Subtract background (50 px rolling ball radius, light background, sliding paraboloid)

  4. Image> AdjustThreshold (Auto)

  5. Analyze Particles (Show outline)

We’re interested in counting the number of crystals and the average size in each photo. Any help would be appreciated! 

u/elixirelyse — 8 days ago