My lab is currently troubleshooting HPLC issues, and one big issue we have run into is that our HPLC will continuously run despite there being a shutdown protocol in the batch file.
Prior to this, the HPLC would, as expected, stop completely after running the shutdown method file during batch runs. However, about a month ago (with no changes to the shutdown protocol file), it began to run continuously, ultimately leading to it running dry. We are stumped because we have not changed any of our methods files, yet this issue has randomly popped up.
We are using a Shimadzu LC-20AD solvent delivery module and a very outdated version of LabSolutions (the computer is not connected to the internet, and we inherited everything from another lab; everything is from ~2012). Does anyone know what could be going on? Could it be an issue of communication between the computer and the pump?