Seeking a prep of long (~150kb) DNA for coauthorship
Trying not to dox myself here, nor do I need to troubleshoot, but we're working on publishing an in-demand technique that requires using loooong DNA for verification, and I wasn't up to buying all the stuff to purify superlong DNA.
If you think you can provide a prep of 10-50 ug of ~150kb (+/- 50kb) linear DNA at around 50-200ng/uL, we could totally use you on this paper! Maybe someone who works with BACs, etc? Because it needs to be linear, you'd have to have a unique cut site (I think some BACs come with homing endonuclease sites, or maybe some have unique RE's?).
The prep would have to be made with kid gloves: no vortexing, no freezing, only gentle pipetting with wide-bore or cut tips, no sidelong glances, and generally no bad energy in the DNA-breaking spectra. Super duper bonus if you could show us a PFGE run with a pure band!
Any takers? Pub should be fairly high reach! Please DM!