
I always loved this shot. It perfectly conveys Vergil's feelings towards his mother and why he is the way he is.
All these years later, he still loves and misses her. Perhaps there was some resentment over her dying protecting Dante before she could get to him but Vergil at this point is wise enough to know it wouldn't have made a difference - because Eva was human and the human body is fragile.
That's why Vergil rejected his human half for as long as he did - because he feared it would make him fragile too and that would mean he'd get hurt again. And that is also why he never allowed anyone to get close to him because they might get hurt if he was not strong enough to protect them - just like with his mother.
As he told Dante seconds earlier, "Might controls everything, and without strength, you cannot protect anything. Let alone yourself." - he wasn't just talking to his brother but reaffirming everything he believes in to himself.