▲ 25 r/labrats
Is SOC medium really required after competent E.coli transformation ?
I need to transform DH5 alpha competent E.coli with synthesized plasmid via Gibson Assembly. For transformation with the assembled plasmid, the protocol says, before plating, cells need to be incubated in SOC media for 60 minutes. I don't have SOC media at the moment. I am just wondering if I can use other media like LB or 2YT ?
Another question i have is: I digested one of my PCR fragments with DnpI to get rid of plasmid template. Then, I column cleaned the digested PCR product. Is this enough to remove DnPI from the mixture?
u/Specific-Surprise390 — 19 hours ago