u/Simple_Volume_5880

I have this protein which lies above 54kDa .I am getting similar pattern in IgG as well as IP .so i ma not sure whether it worked or not .In Ponceau stain i am getting multiple bands in treatment group of both IgG and Ip.i don’t understand what went wrong , usually i think we should get very few bands or only heavy chains bands.I run my sample in 10 and 8 percent gel for better resolution..i had used protein AG agarose beads and no tagged plasmid.I tried to pull endogenous protein with commercial antibody.please help.I want to send this for mass spectrometry to find interacting protein partner with my desired protein .