u/No-Philosopher3209

I work on small RNAs, about 30 nucleotide long. I want to do in-vitro transcription for which i need to do PCR first. But I am having problem getting the correct PCR prodct when checked in gel electrophoresis.

The forward primer that was used has Tm 60, reverse primer has Tm 57.6. I used Q5 2X mastermix for PCR and 0.5 micromolar primers were used. When i did gel for the PCR products I got too many bands. I did gradient pcr in order to optimize the annealing temperature and got similar results for all of them. I am not sure what went wrong.