Thermocycler issues
Hey everybody! I’ve been trying to work with Collembolan DNA more than two months and my results were low-quality all this time (Even though any ants matrix worked well). Yesterday I noticed strange fluctuations in temperature graph, which were not in the protocols obviously. My thermocycler model is BioRad Tetrad 2 (four blocks) from 2005. So can it be the real problem with temperatures or some sort of a software/graph drawing malfunction? How to distinguish them? Thanks in advance
u/Myrmecologist_ — 7 days ago