Hi,
I am running a RP-HPLC method for the quantification of PS20 using a Corona Veo CAD.
The pic attached is of two injections of the same vial. Both Peak 1 and Peak 2 are components of PS20.
The black line is typical and the blue shows an atypical response for "Peak 1".
We generally have no issues with this method except for an occasional artefact peak that may interfere with peak 1 or 2 but have never generated a peak like this.
The black line is the first injection of the two. And there were ten injections in between them. No issues with any other injections on the run.
Column: C18 5um 300Å
MPA: Water w/ 1% Formic acid
MPB: ACN w/ 1% Formic acid
The question is, would anyone have any hypotheses on what may have caused the difference between injections? Any help would be greatly appreciated.