u/Ladr0nCaguai

Solved. Sampler got connected succesfully. Now i'm doing tests. I wish i wasn't that underpayed :(

Hi there! Hope you are doing well. Last week i asked about advice changing the gripper arm in an Agilent G1313A autosampler. Unfortunately my boss said it was better to change all the autosampler (G1329A) instead of the gripper arm.

But, as soon they connected it, OpenLabCDS doesn't recognize it.

Here is an example of what appears in the layout:

https://preview.redd.it/wuli9epcdn0h1.png?width=1513&format=png&auto=webp&s=5c59df052725de32b58b04a84938904aedb493db

I'm not an expert of software development, but LabAdvisor shows me sampler and system firmware isn't the same.

https://preview.redd.it/u5qet88jdn0h1.png?width=1920&format=png&auto=webp&s=78dec65c04cc4cf0e837e87d4e3144e74a292590

Is this the reason why OpenLab doesn't detect it? Sampler is on, and connected.

Thanks in advance! :)

Hi! It's me again. :)
Unfortunately the gripper arm of the Agilent that i use broke one of its fingers.

I've been trying to replace the gripper arm with a new one, as it shows the Agilent Manual. But, when I rotate it to the left in the final step, it doesn't get installed. The steps that I'm following are:

1.Put the clip/screwdriver in the latch and hold it.

2. Push-up the gripper.

3. Rotate it to the left.

Or, perhaps, do you have a videotutorial showing how to change it? Thanks in advance! :)

Agilent gripper arm replacement

One of the Agilent's at work

So, i've been working with a method validations these days. I set all my mobile phase (purged, filtered and degasified), changed the frit and washed the column. Both phases are Acetonitrile HPLC and Acidic water (pH 3.0) with 0.69g NAH2PO4 / L, with Gradient, at 50°C. I started the running sequence, last two days everything went normal but my coworker sent me a message showing me that back pressure stopped the analysis.

I reversed the column, tried to wash it with acidic water with methanol 85:15, but it doesn't show any change.

The column is new, i don't understand how could this happen, any ideas? I've been looking for dissolve "salts" that might be inside the column, but back pressure won't let me wash them away (if there are any salts inside). Column is not connected to Detector, for safety