u/Justmenoworries422

Pharma and First Aid (Transformers: More than Meets the Eye): Pharma is a slimy, selfish weasel of a medic, whose crimes include euthanizing and killing patients so he could harvest and sell their organs and creating a plague that he proceeded to set loose in a medical facility. Years after that, his fellow medics and former friends find him working for a religious zealot who is planning a genocide. First Aid wants to kill him after Pharma kills his friend Ambulon, but Ratchet argues against it, saying if they do that, Pharma "wins". First Aid later corners Pharma and not only does Pharma show zero remorse for what he's done, he has the gall to make fun of Ambulon's death. First Aid snaps and blows Pharma's head clean off, finally putting an end to his atrocities.

Deadpool and Francis (Deadpool): Francis subjected Wade to extremely torturous experiments, leading to him getting disfigured in the process. Wade spends the entire movie hunting him down so he can reverse it. Once Wade has him dead to rights, Francis laughs in his face and tells him he can't fix him. Colossus then appears on the scene, giving a dramatic speech about heroism and sparing your enemies. Wade doesn't even let him finish before he puts a hole in Francis' head.

Mark and Onaan (Invincible): Onaan attacks Mark's family and tries to kill his daughter Terra. Mark later uses him in a hostage exchange with Onaan's father Thragg, an intergalactic tyrant. Thragg tries to call Mark's bluff, saying that a hero like Mark wouldn't just kill a helpless hostage. To which Mark replies that "being a hero is bullshit" and murders Onaan right then and there.

Kate Cha/Dupli-Kate (Invincible): Due to an ancestral curse, Kate has the power to clone herself indefinitely. Every Kate can feel what the others feel and because Kate is a superhero, that means she's felt herself get mutilated, torn apart, shot, killed and otherwise brutalized several times. It gets so bad she eventually decides to retire as she's become traumatized by repeatedly dying.

Jin Bubaigawara/Twice (My Hero Academia): Jin can create copies of everyone and everything, including himself. In the past he coped with his loneliness by cloning himself for company. This was a bad idea, as the clones eventually startet arguing about which of them was the real Jin, resulting in them slaughtering each other to prove themselves as the original. In the end, only one Jin remains, unsure whether he's the original or not.

Copperhead's "Escape" (Justice League): During a brawl between the League and several villains, Copperhead jumps on Hawkgirl's back and threatens to poison her if she doesn't fly him away. Hawkgirl obliges, only to suddenly stop once they're at a high altitude. Copperhead reiterates his threat, only for Hawkgirl to point out that if he kills her now, he'll fall to his death. He's knocked out and arrested shortly after.

Con-artist picks the wrong marks (The Owl House): A scammer spreads false rumors about a mythical flower that grants immortality, so he can drain the life from his unwitting targets. Unfortunately for him, said targets in this case are Eda, the most powerful witch in the Boiling Isles and her sister Lilith, the head of the Emperor's Coven. He gets the snot beaten out of him the second the gig is up.

For me it's a tie between Aphrodite and Hecate.