Yol know any resources where I can theoretically study about flow cytometry - protocols, uses, optimisations and troubleshooting at a beginner to moderate level?
I wanna have as much information as possible before I get into my job.
We’d be using flow cytometry during car - T manufacturing.
Hi everyone,
I’m currently building my understanding around CAR-T cell therapy manufacturing, specifically in the context of process development, GMP translation, and clinical manufacturing workflows for AML indications.
I’m particularly interested in connecting with people working on:
• CAR-T **process development (PD / CMC)**
• GMP manufacturing / cleanroom production
• tech transfer from research → GMP
• analytical QC / release testing
• scale-up / scale-out strategies (autologous or allogeneic)
From what I’ve been reading, the biggest bottlenecks seem to be:
• variability in starting T-cell material
• exhaustion driven by long manufacturing timelines
• tight GMP constraints vs experimental flexibility
• and the difficulty of translating “good biology” into reproducible GMP processes
I’d really appreciate hearing from anyone actually working in this space about:
• what your day-to-day process development looks like
• where most manufacturing failures or delays actually happen
• what skills matter most for transitioning into GMP/CMC roles
• and how teams are solving AML-specific challenges (antigen selection, microenvironment resistance, etc.)
I’m not looking for job leads—just trying to understand the real-world workflow beyond papers and reviews, and connect with people in the field.
Happy to chat here or move to DMs if more convenient.