u/Ill_Chipmunk9002

Problem to link gene ID RNA-seq with CHIP-seq data

Hellow guys, I'm a newbie at bioinformatics.

I'm trying to integrate RNA-seq Kallisto data with my targets that I got from CHIP-seq. But, I have a big problem:

My ORF ID are in different model between the files. While my RNA-seq ID is sequencial orf index (ucsf_hc.01_1.G217B.00001 , ucsf_hc.01_1.G217B.00002, ucsf_hc.01_1.G217B.00003 ...), my targets are genomic coordinate (JAEVHH end_cordinate.start_cordinate). I tried to use a ORF.gff file to link sequencial index with the coordinate, but it doesn't have both information to link.

Someone could help me find out an alternative that I can follow.

Thanks for any contribution!!

reddit.com
u/Ill_Chipmunk9002 — 2 days ago

Problem with link gene ID from RNA-seq and CHIP-seq

Hellow guys, I'm a newbie at bioinformatics.

I'm trying to integrate RNA-seq Kallisto data with my targets that I got from CHIP-seq. But, I have a big problem:

My ORF ID are in different model between the files. While my RNA-seq ID is sequencial orf index (ucsf_hc.01_1.G217B.00001 , ucsf_hc.01_1.G217B.00002, ucsf_hc.01_1.G217B.00003 ...), my targets are genomic coordinate (JAEVHH end_cordinate.start_cordinate). I tried to use a ORF.gff file to link sequencial index with the coordinate, but it doesn't have both information to link.

Someone could help me?

reddit.com
u/Ill_Chipmunk9002 — 2 days ago

Help me on my work

My targets from CHIP-seq

RNA-seq base data

ORF.gff file ; last column is translation gene

Hellow guys, I'm a newbie at bioinformatics.

I'm trying to integrate RNA-seq Kallisto data with my targets that I got from CHIP-seq. But, I have a big problem:

My ORF ID are in different model between the files. While my RNA-seq ID is sequencial orf index (ucsf_hc.01_1.G217B.00001 , 00002, 00003...), my targets are genomic coordinate (JAEVHH*****). I tried to use a ORF.gff file to link sequencial index with the coordinate, but it doesn't have both information to link.

Someone could help me?

reddit.com
u/Ill_Chipmunk9002 — 3 days ago