u/GeneRider

My lab is trying to recover B cells from human spleen, and it's been really tough to get cells in good condition. This may be because of delays in tissue isolation and shipping post mortem. After isolation we have barely got any cells that look intact (based on live/dead staining and flow).

Does anyone have lessons learned, or recommendations for cell dissociation and recovery methods? We are primarily looking to use the cells in flow and RNA-Seq.