traumatized after working 12 hours
i want to preface this by saying that i love my job and its my first full time job so i might just not be used to this (started full time a month ago).
i do research at a university affiliated lab and my PI prepared 50 384-well plates for me and my coworker to transfer the supernatent from and add a few more reagents. so we were dealing with 100 plates total.
we only had one multichannel pipette which we needed to pipette anything in or out of the wells. after swapping back and forth taking turns for the first 15 plates (which took us 3.5 hours), me and my coworker realized that i was way faster at pipetting, so i did all the remaining 35 plates while she was helping me out by pouring time sensitive reagents into reservoirs and sealing my plates ect. we ended up finishing at 8:45 pm, meaning i was at work for around 12.5 hours
im a girl and i was really tired yesterday because i got my period at work and didn't have any medication (i cramp really bad). i also didn't exactly have breakfast that morning because i overslept and i didn't know i would be at work for that long because i am unfamiliar with the protocol and my PI didn't give us any warning despite her knowing we only had one multichannel pipette, so i had one meal between the time i woke up at 9 pm when i got home. i was so tired, hungry, and exhausted by the end of the process that i started crying a little while preparing the plates.
my PI is out of town and she sent us an email asking how it went and i responded to her by saying that the plates are ready but we were still at the lab and it was an incredibly difficult process for us to do with just one pipette. she responded with "Great! Don't forget to lock all the doors before you leave." That kind of really disturbed me and I cried on the drive home because i miss my dad and my friends and my ex partner who i broke up with 3 weeks ago (i moved to a new city for this job so no one is around), and my plans to have a nice calm Friday after a busy week were all gone. i was also worried about my kitten at home.
thank u for listening