u/Crow0911

Hi all,

I'm new to doing analysis on high-dimensional cytometry. For using the unsupervised clustering like FlowSOM or phenograph, may I ask how would you guys verify the identity of the cluster? I saw a tutorial on analyzing mass cytometry data, saying that using the heatmap would be sufficient, but i'm a little bit hesitate on that.

The mass cytometry tutorial is attached here: https://nbisweden.github.io/single-cell_sib_scilifelab/session-cytof-differential/cytof_workflow.html

Thank you! :)