u/CartierRose

Hi all,

Looking for some guidance here. I have some stimulations I need to do on Rorgt-eGFP positive cells (mice). The ideal situation for me would be to stimulate all leukocytes and run intracellular cytokine staining for IL-22.

Of course with GFP permeabilisation becomes difficult. I was wondering if anyone had any experience in this or has done something similar before? Has anyone tried this using the BD cytoperm/cytofix kit? It’s a transcription factor so I feel it might be okay relative to a cytosolic protein.

My backup plan is to sort but these cells are so rare I think this will be difficult.

Thanks!