u/Capable-Club8057

So i have a plasmid with a T7RNAP expression cassette I want to clone into another plasmid. The donor plasmid has a lacI site both inside and outside the cassette I ultimately want to clone. So to get rid of that, I am doing a double digest > agarose > gel purification to exclude that unwanted lacI, leaving just the PORTION of the original plasmid that contains the T7RNAP cassette.

Once I PCR this portion of the original plasmid that contains the T7RNAP system (fragment of interest), do I need to use Dpnl to ensure the methylated DNA from the gel purification is not potentially carried over to down stream transformation? Thank you!