Validating untargeted metabolomics results
Hello everyone,
I would like to discuss something related to untargeted metabolomics(UTM) . I have not read in detail about this topic so i would like your opinion on this. I have heard that the annotation of metabolites based on their retention time and peak alignment could be merely prediction, there are chances that the metabolite annotated could be some other metabolite in reality. So, in that case, how do i validate my UTM results? Targeted metabolomics is mostly what people use for validating, but what about some other techniques? like how proteomics results could be validated by western blots. You could see the results on the blot if it is up/down regulated. Can ELISA or any colorimetric assay help? But kits for many metabolites wont be available. So, how to address this problem.
Any suggestion is most welcomed.