u/Alchemist464

Hello,

I have recently been using the integrating sphere on an Edinburgh SF5 spectrofluorometer and am not getting accurate measurements. I was testing a sample of Rhodamine 6G in ethanol which has a QY of 0.95 ( max abs: 530nm max ems: 552 nm)

I let the lamp warm up for 15 min, made a solution with an optical density of 0.1, and excited the solution at 485 nm. I set the excitation bandwidth at 4.5 nm and emission bandwidth at 0.23 nm. I previously ran with slightly different settings and the QY was very variable (like 0.60 and 0.70).

Are there any spectroscopy pros with advice on how to get accurate and precise QY values.

Thank you!